Sunday, 28 September 2014
Can I Eat That
Abstract
The objective: The purpose of my research was to see if there are foods that are potentially dangerous to neutropenic patients that are not listed on the food
guides they are given by their doctors. I was most interested in the foods routinely consumed by teenagers that had been opened and used by multiple users. These foods
would be studied for the presence of bacteria.
Methods/Materials
Potentially harmful foods and test foods were studied. Using sterile technique I placed 1 ml of each food item into 10 ml of thioglycollate broth, incubated for 18
hours 35*C. then cultured .001 ml onto agar plates. The plates were incubated for 72 hours then observed for the presence of different colony types and the colony
counts in colony forming units(CFU) were determined per ml of each food item.
Results
All potentially harmful foods grew up to four different bacteria with CFU ranging from 1,000,000 CFU to 3,500,000 CFU. Eleven of the thirteen test foods grew 1 to
3 different bacterial species ranging from 1,000,000 to 2,500,000 CFU.
Conclusions/Discussion
Both potentially harmful food and test foods grew high counts of bacteria. Growth of bacteria in test foods was related to (1)poor protection of the product during
storage, (2)many entries into a product by many users, (3)the product being direct from nature and (4)nutrients present in the food that could support the growth of
bacteria. Four recommendations were developed to guide teenagers undergoiing chemotherapy make safer food choices.
This experiment studied bacterial contamination in food items often consumed by teenagers to see if they would be safe to consume while neutropenic from
chemotherapy.
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